HM74, also known as PUMAG or Puma-g, is a member of the G protein coupled receptor (GPCR) superfamily.
In humans, HM74 is encoded by two different genes (GPR109A and GPR109B) that produce proteins, namely HM74A and HM74 (or HM74B), which are 96% homologous.
In mice and rats, only one gene encodes the HM74 protein (Gpr109a).
HM74 is a G(i) protein-coupled receptor that mediates the metabolic effects of nicotinic acid.
Localizing to the cell membrane, HM74 is highly expressed in adipocytes, immune cells and spleen.
Like all members of the GPCR superfamily, HM74 contains seven transmembrane domains.
HM74 lacks the N-linked glycosylation sites near the N-terminus that are present in other GPCR family members.
Applications:Suitable for use in Immunofluorescence, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes endogenous levels of human HM74. Species Crossreactivity: mouse, and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 300-350 of human HM74
