84-2346-32　［取扱停止］HLA-DOalpha (HLA class II histocompatibility Antigen, DO alpha Chain, MHC DN-alpha, MHC DZ alpha, MHC class II Antigen DOA, HLA-DNA, HLA-DZA, HLA-DOA) 100ul　223301

特徴

• Reacts with HLA-DO, a heterodimer formed by DNalpha and DObeta subunits in B cells.
• DNalpha and DObeta are the products of the non-classical class II genes, HLA-DNA and HLA-DOB, respectively.
• DO forms tight complexes with DM in the endoplasmic reticulum and is thereby sorted to lysosomal vesicles during antigen processing and presentation.
• It is tightly associated with DM and it is selectively expressed on antigen-presenting cells, such as B cells, dendritic cells and thymic epithelial cells.
• DO can enhance the efficiency of peptide loading and has been found to stabilize DM at low pH, preserving its chaperon activity.
• DO-DM complexes are more efficient than DM in protecting empty DR molecules.
• Reports describe DO as a co-chaperone of DM.
• This antibody has also been reported to be suitable for western blot and immunoprecipitation.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.