Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes.
The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin.
The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination.
These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression.
Applications:Suitable for use in Western Blot and Immunofluorescence/Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:2000 Immunofluorescence/Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography. >95% by SDS-PAGE.
Form：Supplied as a liquid PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of Histone H4 (Di-Methyl at Arg3) at ~11kD. Species Crossreactivity: human, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：ChIP IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to Histone H4.
