Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes.
The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin.
The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination.
These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression.
In most species, Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry, CHIP.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200 Immunofluorescence: 1:50-1:200Immunoprecipitation: 1:50-1:200 CHIP: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of Histone H3 (Mono-Methyl K4). Species Crossreactivity: Human, Mouse, Rat, Other (Wide Range)
Isotype：IgG
Calc Applications Abbrev：ChIP IF IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide conjugated to KLH derived from within residues 1-100 of Human Histone H3, mono methylated at K4.
