In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure.
The octamer is composed of histones H2A, H2B H3 and H4 and it associates with ~200 base pairs of DNA to form the nucleosome.
The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA.
Histone H1 is required for the condensation of nucleosome chains into higher order structures.
Phosporylation of Histone H1 is thought be involved in this process, although the exact nature of this role has yet to be elucidated.
Evidence suggests that Histone H1 is a part of a general repressor mechanism for stable repression of transcription, but it can also activate transcription of specific genes.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Histone H2B. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the N-terminal of Human Histone H2B.
