Eukaryotic Histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers.
Two molecules of each of the four core Histones (Histone H2A, H2B, H3, and H4) form the octamer, which consists of two H2A-H2B dimers and two H3-H4 dimers that are nearly symmetrical by tertiary structure.
Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin.
H1FOO (H1 histone family, member O, oocyte-specific), also known as OSH1, is a 346 amino acid oocyte-specific Histone that localizes to both the nucleus and the cytoplasm.
Expressed as multiple alternatively spliced isoforms, H1FOO is thought to play an important role in gene control during oogenesis and early embryogenesis and is crucial for meiotic maturation of germinal vesicle-stage oocytes.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Histone H1oo. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the N-terminal of Human Histone H1oo.
