Histone H1.0 (H1 histone family, member 0) is a lysine rich member of the H1 family of linker histones.
The H1 family of proteins interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin.
Histone H10 is a unique variant considered a replacement linker histone which is expressed and incorporated into chromatin in the absence of DNA replication.
In contrast, the majority of somatic H1 histones are replication dependent variants found in proliferating cells.
Histone H1.0 is expressed in cells that are in the terminal stages of differentiation or that have low rates of cell division.
Unlike other differentiation-specific linker histones which demonstrate tissue and species-specific expression, Histone H1.0 is widely expressed in many tissues in most vertebrates.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Histone H1.0. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 61-110 of Human Histone H1.0.
