Histamine is an inflammatory mediator that is ubiquitously expressed and has a broad range of pharmacologic effects.
Specifically, it plays a role in the central nervous, gastrointestinal, respiratory and immune systems.
The effects of histamine are mediated by a family of G protein-coupled receptors,the Histamine H1, H2, H3 and H4 Receptors.
The gene encoding the human Histamine H3 Receptor is located on chromosome 20 and is expressed as six alternative splice variants in thalamus.
These isoforms contain either a deletion in the second transmembrane domain or a variable deletion in the third intracellular loop.
The existence of multiple H3 Receptor isoforms suggests that H3-mediated effects may be regulated through alternative splicing mechanisms.The H3 Receptor acts as an autoreceptor in the central nervous system (CNS) and modulates histamine synthesis and release.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 15mM sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Histamine H3. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 300-350 of Human Histamine H3 Receptor.
