Histamine is an inflammatory mediator that is ubiquitously expressed and has a broad range of pharmacologic effects.
Specifically, it plays a role in the central nervous, gastrointestinal, respiratory and immune systems.
The effects of histamine are mediated by a family of G protein-coupled receptors, the Histamine H1, H2, H3 and H4 Receptors.
The gene encoding the human Histamine H1 Receptor maps to chromosome 3p25 and is expressed in highest abundance in placenta, with lower levels in lung, skeletal muscle, kidney and brain.
The murine Histamine H2 Receptor gene maps to chromosome 13 and is highly expressed in stomach with moderate expression in brain and heart.
The gene encoding the human Histamine H3 Receptor is located on chromosome 20 and is expressed as six alternative splice variants in thalamus.
Applications:Suitable for use in Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunofluorescence: 1:50-1;200Immunohistochemistry: 1;50-1;200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes endogenous levels of human Histamine H1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa150-200 of human Histamine H1 Receptor
