The Homeodomain-Interacting Protein Kinase (HIPK) family, which includes HIPK1, HIPK2, HIPK3, contains a conserved protein kinase domain as well as a separate domain that interacts with homeoproteins.
HIPK2, the most highly characterized family member, is thought to act as a co-repressor of homeodomain transcription factors as HIPK2 has been shown to enhance the DNA binding of the NK-3 homeoprotein in vitro.
It is regulated by a posttranslational modification of a ubiquitin-like protein, SUMO-1, via covalent bonding to a lysine residue on HIPK2.
This is similar to the binding of SUMO-1 to PML and Sp100.
The conjugation of SUMO-1 is thought to direct each of these proteins to nuclear bodies (NBs), which appear to play a role in autoimmunity and viral protection.
HIPK2 is the first protein kinase to be directed to nuclear bodies in response to ubiquitin-like modification.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of HIPK2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human HIPK2.
