HiNF-P is a critial transcription factor which is necessay for E2F-independent activation of the Histone H4 multigene family.
HiNF-P associates with conserved H4 cell cycle regulatory sequences in vivo.
Antisense inhibition of HiNF-P reduces endogenous Histone H4 gene expression.
HiNF-P utilizes NPAT/p220, a substrate of the cyclin E/cyclin-dependent kinase 2 (CDK2) kinase complex, as a crucial coactivator to amplify Histone H4 gene transcription.
The biological role of HiNF-P is reflected by impeded cell cycle progression into S phase upon antisense-mediated reduction of HiNF-P levels.
Research indicates that HiNF-P is the key link in a linear signaling pathway that is initiated with the growth factor-dependent induction of cyclin E/ CDK2 kinase activity at the restriction point and culminates in the activation of Histone H4 genes through HiNF-P at the G1/S phase transition.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of HINFP. Species Crossreactivity: Human, mouse
