84-2344-89　［取扱停止］HIF1AN (Factor inhibiting HIF-1, Factor inhibiting HIF1, FIH 1, FIH-1, FIH1, FLJ20615, FLJ22027, HIF1AN, HIF1N_HUMAN, Hypoxia inducible Factor 1 alpha Inhibitor, Hypoxia inducible Factor 1 alpha Subunit Inhibitor, Hypoxia inducible Factor asparagine hydroxylase, Hypoxia-inducible Factor 1-alpha Inhibitor, Hypoxia-inducible Factor asparagine hydroxylase, Peptide aspartate beta dioxygenase,) 50ul　223222

特徴

• Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha.
• Specifically, FIH-1 operates as an asparaginyl hydroxylase.
• It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha.
• This hydroxylation event blocks the association of HIF-1alpha with co-activators.
• FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha.
• In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm.
• The structure of FIH-1 includes a jellyroll-like beta-barrel containing ferrous-binding triad residues.
• The gene encoding human FIH-1 maps to chromosome 10q24.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.