Cell growth and viability is compromised by oxygen deprivation (hypoxia).
Hypoxia-inducible factors, including HIF-1alpha, Arnt 1 (also designated HIF-1beta), EPAS-1 (also designated HIF-2alpha) and HIF-3alpha, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis.
Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family.
In response to hypoxia, HIF-1alpha is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex.
The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of HIF-1alpha. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 345-398 of Human HIF-1α.
Shelf Life：1year
Swiss Prot Number：Q16665
EU Commodity Code：30021010
商品名：HIF-1alpha (ARNT interacting Protein, ARNT-interacting Protein, Basic helix loop helix PAS Protein MOP1, Basic-helix-loop-helix-PAS Protein MOP1, bHLHe78, Class E basic Helix-Loop-Helix Protein 78, HIF 1A, HIF 1alpha, HIF-1-alpha, HIF1 A, HIF1 Alpha, HIF1-alpha, HIF1A, HIF1A, Hypoxia inducible Factor 1 alpha, Hypoxia inducible Factor 1 alpha Isoform I.3, Hypoxia inducible Factor 1 alpha Subunit, Hypoxia inducible Factor 1 alpha Subunit basic helix loop helix Transcription Factor, Hyp) 100ul
