84-2344-60　［取扱停止］HES-6 (Transcription Cofactor HES-6, C-HAIRY1, Class B basic Helix-Loop-Helix Protein 41, bHLHb41, Hairy and Enhancer of split 6, BHLHB41, HES6) 100ul　223206

特徴

• The HES family members form a complex with TLE, the mammalian homologue of Groucho, and this interaction is mediated by the carboxy terminal WRPW motif of the HES proteins.
• The HES/TLE complex functions by directly binding to DNA, instead of interfering with activator proteins.
• Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) as opposed to the E box (CANNTG).
• HES2 binds to both N and E box sites, while HES6 does not bind DNA.
• Rather, HES6 inhibits HES1 activity, thereby promoting transcription.
• HES1 and HES2 are expressed in a variety of adult and embryonic tissues.
• HES3 is expressed exclusively in cerebellar Purkinje cells, and HES5 is found solely in the nervous system.
• HES6 is produced in brain as well as in the limb buds of developing embryos.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.