The helix-loop-helix (HLH) structures are known motifs commonly found in membrane-active and DNA-binding proteins.
Helix-loop-helix proteins (HEN1 and HEN2) are DNA-binding proteins that may be involved in cell-type determination in the early nervous system.
Studies of expression in normal tissues have demonstrated expression of NSCL-1 and NSCL-2 (genes encoding HEN1 and HEN2) in the developing central and peripheral nervous system, specifically in developing neurons.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity:Recognizes endogenous levels of HEN1/2. Species Crossreactivity: Human, mouse, rat
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Synthetic peptide corresponding to amino acids 50-100 of Human HEN1.