84-2344-45　［取扱停止］HEC1 (Kinetochore Protein NDC80 Homolog, Highly expressed in Cancer Protein, Kinetochore Protein Hec1, HsHec1, Kinetochore-Associated Protein 2, Retinoblastoma-Associated Protein HEC, NDC80, HEC1, KNTC2) 50ul　223199

特徴

• Highly expressed in cancer (Hec1) is a coiled-coil-enriched protein expressed abundantly in the S and M phases of rapidly dividing cells where it localizes to the kinetochores.
• Hec1 is involved in spindle checkpoint signaling.
• Hec1 is not expressed in terminal differentiated cells.
• Hec1 is expressed in tissues with high mitotic rates including testis, spleen and thymus.
• Hec1 is also found in the late S to M phases of bladder carcinoma cells.
• In dividing cells, Hec1 is required for the recruitment of Mps1 kinase and MAD1/MAD2 complexes to the kinetochores.
• The phosphorylation of Hec1 on Serine 165 by Nek2 is essential for faithful chromosome segregation.
• The binding of retinoblastoma protein to Hec1 also increases the fidelity of chromosomal segregation.
• Applications:Suitable for use in Immunofluorescence, Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.