Src is the human homolog of the v-src gene of the Rous sarcoma virus, also called avian sarcoma virus or ASV.
Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human.
By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk.
Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility and adhesion.
Src family kinases contain an amino terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively.
Src family kinases are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis.
Different subcellular localizations of Src family kinases may be important for the regulation of specific cellular processes such as mitogenesis, cytoskeletal organization and membrane trafficking.
The human hemopoietic cell kinase (Hck) gene maps to chromosome 20q11-q12 and encodes a 505 amino acid protein.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of HCK. Species Crossreactivity: Human, mouse, rat
