HCCA2 (Hepatocellular carcinoma-associated gene 2), also known as MOB2 (Mps one binder kinase activator-like 2), is a 467 amino acid protein that belongs to the MOB1/phocein family.
HCCA2 is expressed in lung, spleen, brain, and fetal liver.
It is highly expressed in hepatocellular carcinoma.
HCCA2 is localized in the perinuclear region of the cytoplasm in liver cancer tissues and colocalizes with MAD2L2 in the nucleus of Hela cells.
HCCA2 is chracaterized by two N-glycosylation sites, six N-myristolylation sites, two Src homolgy 3 (SH3), and several phosphorylation motifs which indicate that this protein may play a role in an intracellular signal transduction cascade.
HCCA2 binds to and regulates the autophosphorylation of the related human serine/threonine kinase 38 (NDR1) and serine/threonine kinase 38L (NDR2).
It has been shown that HCCA2 plays a critical role in cell cycle regulation.
Overexpression of the protein during the G0/G1 phase inhibits cell proliferation causing cell cycle arrest.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of HCCA2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human HCCA2.
