Heparin binding epidermal-like growth factor (HB-EGF), a member of the EGF family of mitogens, binds to the EGF receptor (EGFR) and to heparin sulfate proteoglycans on the cell surface.
HB-EGF was originally isolated from medium conditioned by the growth of the human histocytic lymphoma cell U-937 on the basis of its heparin-binding ability and its mitogenic activity for Balb-3T3 fibroblasts.
The HB-EGF gene encodes a 208 amino acid precursor containing a signal peptide and transmembrane domain.
Mature HB-EGF is a soluble protein 86 amino acids in length and results from the enzymatic cleavage of the membrane bound precursor.
The membrane-bound form of HB-EGF has been identified as the diphtheria toxin receptor.
Preincubation of Vero cells with phorbol 12-myristate 13-acetate (PMA) induces the proteolytic cleavage of HB-EGF outside the membrane anchor.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of HBEGF. Species Crossreactivity: Human, mouse, rat
