84-2343-42　［取扱停止］HAO1 (Hydroxyacid Oxidase 1, HAOX1, Glycolate Oxidase, GOX, HAO1, GOX1, HAOX1) 50ul　223146

特徴

• HAO1 (hydroxyacid oxidase 1) is also known as GOX1 (glycolate oxidase 1) or HAOX1 and is a 370 amino acid protein that is expressed in liver and pancreas.
• HAO1 is localized to peroxisomes and aids in organic acid metabolism via 2-hydroxyacid oxidase activity.
• 2-hydroxyacid oxidases, such as HAO1, are enzymes that require a flavin cofactor to oxidize 2-hydroxyacids to 2-ketoacids while reducing oxygen to hydrogen peroxide.
• HAO1 prefenentially oxidizes the substrate glycolate and also oxidizes other substrates, including 2-hydroxy fatty acids as well as L-å-hydroxy acids of moderately short chain lengths.
• The oxidation of glycolate yields glyoxylate which is utilized for peroxisomal synthesis of glycine.
• HAO1 is also able to convert glyoxylate to oxalate.
• HAO1 is thought to play a role in the pathophysiology of hyperoxaluria type 1, which is caused by defects in AGXT, a peroxisomal enzyme, leading to accumulation of glyoxylate.
• Hyperoxaluria type 1 is characterized by an accumulation of oxalate that is thought to lead to precipitates of calcium oxalate in kidneys which can be fatal.
• Applications:Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:100Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.