84-2343-24　［取扱停止］GZMA (CTL tryptase, CTLA3, Cytolytic t Cell and Natural Killer Cell specific trypsin like Serine Protease, Cytotoxic T Lymphocyte Associated Serine esterase 3, Cytotoxic T Lymphocyte Proteinase 1, Cytotoxic T-Lymphocyte Proteinase 1, Fragmentin 1, Fragmentin-1, GRAA_HUMAN, Granzyme 1 cytotoxic T Lymphocyte Associated Serine esterase 3, Granzyme A (Cytotoxic T Lymp ...　223134

特徴

• Granzyme A and granzyme B are serine proteases that mediate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells.
• Both granzyme A and granzyme B are synthesized as inactive proenzymes, and they are stored within cytolytic granules and released by effector cells during degranulation.
• In activated CTLs, granzyme A and granzyme B are processed and activated by cathepsin C, and they then function to induce apoptosis by two distinct pathways.
• Granzyme B proteolytically cleaves and activates members of the caspase family of cysteine proteases, including caspase-3, caspase-6, caspase-7 and caspase-9.
• When cleaved, these caspases assemble into active holoenzymes that then mediate apoptosis through a defined proteolytic cascade involving nuclear lamins and PARP (poly (ADP ribose) polymerase).
• Granzyme A mediates the activation of apoptosis by inducing single-strand DNA breaks, membrane perturbation and nuclear condensations in an alternative pathway that is independent from caspase activation or the caspase proteolytic cascade.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.