Glycophorins A, B and C are sialoglycoproteins of the human erythrocyte membrane, which bear the antigenic determinants for the MN, Ss and Gerbich blood groups, respectively.
Glycophorins span the membrane once and present their amino-terminal end to the extracellular surface of the human erythrocyte.
The genetic array of expressed glycophorin surface antigens on erythrocytes defines the blood group phenotype of the individual.
The human Glycophorin A gene maps to chromosome 4q31.21, contains 7 exons which are 97% homologous to Glycophorin B, and encodes a 150 amino acid protein.
The human Glycophorin B gene maps to chromosome 4q31.21 and encodes a 91 amino acid protein.
The human Glycophorin C gene maps to chromosome 2q14.3 and contains four exons.
Glycophorin C transcript can generate two protein isoforms.
Isoform 1 includes all 4 exons and encodes the full length 128 amino acid protein.
Isoform 2 is missing exon 2 and encodes a 109 amino acid protein, which specifies the yus subtype of the Gerbich phenotype.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of GYPA. Species Crossreactivity: Human
