The enzyme beta-glucuronidase catalyzes the conversion of beta-D-glucuronoside and water to an alcohol and D-glucuronate.
Deficiency of beta-glucuronidase is the cause of the human lysosomal storage disorder mucopolysaccharidosis type VII (MPS VII).
Specifically, two residues appear important for catalytic activity: Glu 451 and Glu 540.
Mutations at these sites affect the overall structure of the protein, which normally consists of a homotetramer with each promoter including a jelly roll barrel, an immunoglobulin constant domain and a TIM barrel.
Regulation of beta-glucuronidase activity may play a role in tumorigenesis and the invasiveness of a number of cancers, and is also an important factor in the development of functional prodrugs that require the cleavage of an active cytostatic by endogenous enzymes for antitumor activity.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human GUSB.
