Acrogranin (also designated PC cell-derived growth factor (PCDGF), epithelin/granulin precursor or paragranulin) is a glycosylated protein originally purified from the highly tumorigenic, Insulin-independent mouse teratoma PC cell line.
Acrogranin is a cysteine-rich molecule whose expression is essential for tumorigenicity in teratoma cells.
Acrogranin is expressed in estrogen receptor-positive (ER(+)) human mammary MDA-MB-468 epithelial cells, human breast cancer MCF7 cells and human estrogen-responsive T-47D cells.
Secreted acrogranin acts as an autocrine growth factor for breast carcinoma cells and overexpression may play an important role in human breast cancer.
Acrogranin stimulates the growth of PC cells as well as 3T3 fibroblasts.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of GRN. Species Crossreactivity: Human
