Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist.
This phenomenon, referred to as agonist-mediated desensitization, involves phosphorylation of the receptor by two classes of enzymes.
The first class is comprised of the second messenger-regulated kinases, such as c-AMP dependent protein kinase A and protein kinase C.
The second class includes the G protein-coupled receptor kinases (GRKs).
At least seven members of the GRK family have been identified.
These include rhodopsin kinase (GRK 1), two forms of b-adrenergic receptor kinase: GRK 2 (bARK, bARK1) and GRK 3 (bARK2), IT-11 (GRK 4), GRK 5, GRK 6 and GRK 7.
Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of GRK5. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 350-400 of Human GRK5.
