Growth factor receptor bound protein 2 (GRB2), also known as Ash protein, is a 24kDa protein that contains a central Src homology (SH2) domain flanked by two SH3 domains.
GRB2 is believed to be a regulatory subunit of signaling molecules whose activity is modulated by receptor binding.
GRB2 associates with activated (tyrosine phosphorylated) EGFR and PDGFR via its SH2 domain as well as IRS1, SHC, and LNK through SH2 and SH3 domains.
The SH3 domain binds to SOS, a guanine nucleotide exchange factor for Ras proteins.
Endocytosis of activated EGFR requires the interaction of GRB2 with the GTP binding protein dynamin, a factor essential to the formation of endocytotic vesicle.
It is ubiquitously expressed and several isoforms are produced by alternative splicing.
On SDS PAGE, GRB2 has an apparent molecular weight of 28 kDa.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of GRB2. Species Crossreactivity: Human, mouse, rat
