GPR91 (formerly known as P2U2) is a G protein-coupled, dicarboxylic acid succinate receptor.
It has a high level of expression in the kidney, predominantly in the proximal tubules, and localizes to the plasma membrane.
It has also been found at low levels in the liver and the spleen.
GPR91 functions as a citric acid cycle intermediate succinate receptor.
Two signaling pathways result from GPR91 activation, the pertussis-toxin-sensitive Gi/Go pathway and the pertussis-toxin-insensitive Gq pathway.
Four amino acid residues are necessary for GPR91 activation by succinate: Arg 99, His 103, Arg 252 and Arg 281.
GPR91 plays an important role in the succinate-induced hypertensive effect and may be involved in renovascular hypertension, a disease linked todiabetes, renal failure and atherosclerosis.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.05% sodium azide
Specificity：Recognizes endogenous levels of human GPR91. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IF IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa102-154 of human GPR91. Swiss/UniProt Accession: Q9BXA5
