G protein-coupled receptors (GPCRs) play a central role in mediating the intracellular effects of numerous neurotransmitters and hormones, including melatonin.
GPR50, also designated Melatonin-related receptor, GPCR50 and H9, does not bind melatonin.
It is a multi-pass membrane protein primarily detected in hypothalamus and pituitary.
GPR50 is a 613 amino acid protein that contains the seven hydrophobic segments that are characteristic of GPCRs, as well as the distinguishing sequence motifs of the melatonin receptor GPCR family.
The GPR50 protein is 45% identical to the melatonin receptors 1A and 1B and has an unusually long proline-rich C-terminal tail.
Research suggests that a deletion variant within the GPR50 gene is a sex-specific risk factor for susceptibility to BPAD (bipolar affective disorder) and that other variants in the gene may be sex-specific risk factors in the development of schizophrenia.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 15mM sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of human GPR50. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 345-390 of Human GPR50.
