G protein coupled receptors provide attractive targets for drug therapy due to the sheer size and diversity of ligands within this receptor family.
G proteincoupled receptor 40 (GPR40) functions as a cell-surface receptor for longchain free fatty acids (FFAs).
FFAs provide an important energy source, but also function as signaling molecules in various pathways, notably the process of insulin secretion.
In pancreatic tissue, the interaction of long chain FFAs with GPR40 amplifies glucose-stimulated insulin secretion from beta cells, suggesting a possible role for GPR40 in the treatment of diabetes associated with insulin-deficiency.
Specifically, the Arg211His polymorphism in the GPR40 gene may contribute to the variation of insulin secretory capacity in Japanese men.
Also, GPR40 may be involved in the control of breast cancer cell growth by fatty acids and, therefore, provide a link between fat and cancer.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of GPR40. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 201-250 of Human GPR40.
