Motilin is a widely conserved 22 amino acid peptide hormone secreted from enterochromaffin cells of the gastrointestinal tract.
Within the intestines, motilin potentiates intestinal motility by inducing contractions of the duodenum and through binding to surface receptors, designated GPR38 (G-protein coupled receptor 38).
These motilin receptors are predominantly expressed in stomach, thyroid and bone marrow, and they are related to other G-protein coupled receptors located in the pituitary and hypothalamus, which mediate the signaling for growth hormone secretagogues.
The gene encoding GPR38 is alternately spliced at the carboxy terminus to generate two related proteins that are designated GPR38-A and GPR38-B.
Sequence comparisons of the two isoforms indicate that GPR38-A contains seven transmembrane domains while GPR38-B is predicted to contains only five transmembrane regions.
Consistent with other G-protein coupled receptors, GPR38 activates phospholipase C signal transduction pathways and induces intracellular calcium mobilization after binding of motilin.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of GPR38. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 367-412 of Human GPR38.
