G protein-coupled receptors (GPRs) are a protein family of transmembrane receptors that transmit an extracellular signal (ligand binding) into an intra cellular signal (G protein activation).
GPR signaling is an evolutionarily ancient mechanism used by all eukaryotes to sense environmental stimuli and mediate cell-cell communication.
All of the receptors have seven membrane- spanning domains and the extracellular parts of the receptor can be glycosylated.
These extracellular loops also contain two highly conserved cysteine residues which create disulfide bonds to stabilize the receptor structure.
GPR119 is a 335 amino acid protein that is mainly expressed in the pancreas.
It is an endogenous receptor for lysophosphatidylcholine (LPC), which is is a lipid mediator involved in insulin secretion from pancreatic cells.
GPR119 may participate in this insulin secretion, suggesting that it may be potential target for new anti-diabetic drugs.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of GPR119. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 190-240 of Human GPR119.
