G protein-coupled receptors (GPRs or GPCRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, are members of the largest protein family and play a role in many different stimulus-response pathways.
G protein-coupled receptors mediate extracellular signals into intracellular signals (G protein activation).
They respond to a great variety of signaling molecules, including hormones, neurotransmitters and other proteins and peptides.
GPR proteins are integral seven-pass membrane proteins with some conserved amino acid regions.
G protein coupled receptor 10 (GPR10) acts as a receptor for prolactin-releasing peptide (PrRP).
GPR10 plays a role in the regulation of food intake, pain-signal processing and in lactation.
Primarily expressed in pituitary gland, it is repressed by bromocriptine.
GPR10 interacts with various other proteins, including GRIP1, GRIP2 and PICK1.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of GPR10. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 200-250 of Human GPR10.
