Alpha 1,4-N-acetylflucosaminyltransferase (alpha4Gn-T) mediates the biosynthesis of mucin type glycoprotein (O-glycan).
alpha4Gn-T acts as the key enzyme for the formation of the unique glycan GlcNAcalpha1-4Galbeta1-R, and most efficiently transfers N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans.
alpha4Gn-T is a single-pass type II membrane protein associated with the Golgi apparatus and contains the conserved DXD motif involved in catalytic activity.
It is expressed in stomach and pancreas, as well as in gastric cancer cells.
alpha4Gn-T is not expressed in peripheral blood cells, making it a useful biomarker for pancreatic cancer.
alpha4Gn-T and Mucin 6 expression is upregulated in the gastric mucosa of H.
pylori infected patients, which suggest the involvement of alpha4Gn-T in defense against H.
pylori infection.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of GnTalpha4. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 32-82 of Human GnTα4.
