Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors.
Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (e.g.
adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous.
In mammals, G protein alpha, beta and y polypeptides are encoded by at least 16, 4 and 7 genes, respectively.
Most interest in G proteins has been focused on their alpha subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors.
Evidence, however, has established an important regulatory role for the betay subunits.
It is becoming increasingly clear that different G protein complexes expressed in different tissues carry structurally distinct members of the y as well as the alpha and beta subunits and that preferential associations between members of subunit families increase G protein functional diversity
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of GNG8. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human GNG8.
