Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors.
Each of a very broad range of receptors specifically detects an extracellular stimulus (i.e., a photon, pheromone, odorant, hormone or neurotransmitter), while the effectors (e.g., adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous.
In mammals, G protein a, b and g polypeptides are encoded by at least 16, 4 and 7 genes, respectively.
Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors.
Evidence, however, has established an important regulatory role for the b and g subunits.
The G protein b subunits are important regulators of G protein a subunits as well as of certain signal transduction receptors and effectors.
In mammals, there are five different members of the b subunit family
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of GNB1. Species Crossreactivity: Human, mouse, rat
