Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors.
Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e.
adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous.
In mammals, G protein alpha, beta and y polypeptides are encoded by at least 16, 4 and 7 genes, respectively.
Most interest in G proteins has been focused on their alpha subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors.
Four distinct classes of Galpha subunits have been identified; these include Gs, Gi, Gq and Galpha 12/13.
The Gi class comprises all the known alpha subunits that are susceptible to pertussis toxin modifications, including Galpha i-1, Galpha i-2, Galpha i-3, Galpha o, Galpha t1, Galpha t2, Galpha z and Galpha gust.
Of these, the three Galpha i subtypes function to open atrial potassium channels.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of GNAI1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human GNAI1.
