Glutamine synthetase (GI Syn) forms a homooctamer that serves as a catalyst for the amination of glutamic acid to form glutamine.
This enzyme is a marker for astrocytes, which serve as the primary site of conversion of glutamic acid to glutamine in the brain.
Induction of glutamine synthetase is seen upon astrocyte cell contact with neurons.
Elevated expression of glutamine synthetase in glial cells has been shown to protect neurons from degeneration due to excess glutamate.
Glutamine synthetase is also present in the liver and is involved in nitrogen homeostasis.
Overexpression of glutamine synthetase has been shown in primary liver cancers, indicating a potential role for glutamine synthetase in hepatocyte transformation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of GLUL. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 328-373 of Human GLUL.
