PICOT, also known as GLRX3 (glutaredoxin 3), GRX3, GRX4, GLRX4, HUSSY-22, TXNL2 or TXNL3, is a 335 amino acid protein that contains one thioredoxin domain and two glutaredoxin domains.
Localized to the cytoplasm and the cell cortex, PICOT is thought to play a role in regulating the thioredoxin system and may weakly interact with PKC θ (protein kinase C theta).
Through its ability to regulate the thioredoxin pathway, PICOT inhibits cardiac hypertrophy (a thickening of the heart muscle usually caused by high blood pressure) by negatively regulating NFAT (Nuclear factor of activated T-cells) signaling.
Although PICOT contains one thioredoxin domain, it lacks the two redox-reactive cysteines that are required for catalytic activity, suggesting that PICOT lacks thioredoxin function.
PICOT is expressed in testis, heart and spleen with lower levels detected in thymus, lung, colon, placenta and small intestine.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of GLRX3. Species Crossreactivity: Human, mouse, rat
