In the central nervous system (CNS), glycine-mediated inhibitory neurotransmission is essential to voluntary motor control and reflex responses.
Glycine binds to glycine receptors (GlyR) in the postsynaptic neuronal membranes.
GlyR, gamma-aminobutryic acid, serotonin and acetylcholine comprise an evolutionally conserved superfamily of ligand-gated ion channels.
The pentameric subunit structure of GlyR consists of two types of glycosylated membrane proteins, alpha1 through alpha4 and beta, and an associated peripheral membrane protein, which combine to form a chloride-selective ion channel.
In humans, the composition of the pentamer changes from ?2 subunits in the fetal CNS to alpha1 and beta subunits in the adult CNS.
Fast potentiation of GlyR by intracellular Ca2+ in the brainstem and midbrain indicate an important role for Ca2+ in modulation of glycinergic synapses.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human GLRB. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 252-297 of Human GLRB.
