Glial cell line-derived neurotrophic factor (GDNF) and the related neurotrophic factor neurturin (NTN) are potent survival factors for central and peripheral neurons.
Receptors for these factors include GFRalpha-1 (previously designated GDNFR-alpha), GFRalpha-2 (also designated RETL2), GFRalpha-3 and GFRalpha-4 (also known as persephin receptor).
The receptors do not contain transmembrane domains and are attached to the cell membrane by glycosylphosphoinositol linkage.
Binding of GDNF or NTN to one of these receptors leads to the activation of the tyrosine kinase Ret.
GFRalpha-4 mediates the activation and GDNF-induced autophosphorylation of the RET receptor.
It is mainly expressed in the adult thyroid gland, but lower levels may also be detected in fetal adrenal and thyroid glands.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of GFRalpha-4. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids 148-195 of Human GFRα-4.
