Glial cell line-derived neurotrophic factor (GDNF) and the related neurotrophic factor neurturin (NTN) are potent survival factors for central and peripheral neurons.
GDNF is a glycosylated, disulfide-bonded homodimer that is distantly related to the TGFbeta superfamily of growth factors.
Three receptors for these factors, GFRalpha-1 (also designated GDNFR-alpha, RETL1 or TrnR-1), GFRalpha-2 (also designated GDNFR-beta, RETL2, NTNR-alpha or TrnR-2) and GFRalpha-3 have been identified.
The receptors do not contain transmembrane domains and are attached to the cell membrane by glycosyl-phosphoinositol linkage.
Both GFRalpha-1 and GFRalpha-2 have been shown to mediate the GDNF-dependent and NTN-dependent phosphorylation and activation of the tyrosine kinase Ret.
GFRalpha-3 is expressed only during development.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of GFRalpha-1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 50-100 of Human GFRα-1.
