Growth differentiation factor 15 (GDF-15), also known as PDF, MIC-1, PLAB, NAG-1 or PTGF-beta, is a member of the transforming growth factor beta (TGF-beta) superfamily.
Synthesized intracellularly, the protein is secreted as a dimer linked by disulfide bonds.
Epithelial cells and macrophages are the sites of strongest GDF-15 expression, although it is widely expressed in adult tissue.
In the brain, GDF-15 expression occurs in the choroid plexus, from which the protein is secreted into the cerebrospinal fluid.
The gene for GDF-15 is responsive to p53 tumor suppressor protein, and in cultured cerebellar granule neurons GDF-15 can prevent cell death by the activation of Akt and inhibition of ERK.
GDF-15 acts as a trophic factor for certain classes of neurons, promoting cell survival and differentiation.
Overexpression of GDF-15 occurs in prostate cancer, and may be a means of diagnosis.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of GDF-15. Species Crossreactivity: Human
