Gamma-glutamylcysteine synthetase (y-GCS) is the rate limiting enzyme for glutathione (L-y-glutamyl-L-cysteinylglycine, GSH) synthesis.
GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant.
y-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic ntioxidants, heat shock, oxidants and cytokines.
The human y-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12.
The human y-GCS gene encoding the regulatory subunit maps to chromosome 1p22-p21.
The two subunits of y-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of GCSm-γ. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 50-100 of Human GCSm-γ.
