The GCLC gene consists of 16 exons and encodes the 636 amino acid protein y-GCSc (y-glutamylcysteine synthetase heavy subunit), also designated y-Lglutamate- L-cysteine ligase catalytic subunit (GLCLC).
y-GCSc is expressed in hemocytes, brain, liver and kidney.
y-GCSc associates with a regulatory or modifier subunit, y-GCSm (y-glutamylcysteine synthetase light subunit), to form a heterodimer, y-GCS.
y-GCS is the first enzyme involved and the rate determining step in glutathione iosynthesis.
Oxidants, cadium and methyl mercury upregulate the transcription of y-GCS.
H2O2 regulation depends on the yap1 protein and the presence of glutamate, glutamine and lysine.
Cadium regulates transcription through proteins Met-4, Met-31 and Met-32.
Cbf1, a DNA binding protein, inhibits transcription of y-GCS.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of GCSc-γ. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 300-350 of Human GCSc-γ.
