Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family.
There are two forms of guanylate cyclase, a soluble form (GCS or sGC), which act as receptors for nitric oxide and a membrane-bound receptor form (GC), which are peptide hormone receptors.
The GC-C protein is composed of an extracellular domain, a single transmembrane domain, and a cytoplasmic region consisting of a kinase-like domain and a catalytic domain.
It is expressed as two differentially glycosylated forms, a 130kDa precursor form present in the endoplasmic reticulum and a 145kDa form present on the plasma membrane.
Ligand binding to the extracellular domain of GC-C promotes the accumulation of cGMP.
GC-C acts as the receptor for heatstable enterotoxins, small peptides secreted by some pathogenic strains of E.
coli that cause severe secretory diarrhea.
GC-C also binds to guanylin and uroguanylin peptides, which modulate renal function in response to oral salt load.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 15mM sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of GCS-alpha. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 400-450 of Human GCS-α-1.
