Gene amplification is associated with tumor stage and progression in human gliomas.
Several amplified loci are identified and comprise multiple genes.
The glioma amplified sequence 41 (GAS41) is an evolutionarily conserved eukaryotic protein found in diverse species.
GAS41 is related to the AF-9 and ENL proteins, which are putative transcription factors in some acute leukemias,and interacts with a component of the nuclear matrix, NuMA, in interphase cells.
GAS41 has a dotted staining pattern in interphase nuclei and a uniform distribution in mitotic cells.
GAS41 is ubiquitously expressed, with the highest levels of expression in human brain.
In neuroblastoma, GAS41 is located in the nucleoli, but not in the nucleoplasm.
GAS41 also binds to the MLL fusion partner AF-10, which is involved in two distinct chromosomal translocations associated with hematologic malignancy.
In addition, GAS41 interacts with INI1 (integrase interactor 1), which is a human homolog of the yeast Snf5 protein, a component of the SWI/SNF complex.
The GAS41 gene maps to human chromosome 12q13-q15.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 15mM sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of GAS41. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 11-60 of Human GAS41.
