Gas2 is a 313 amino acid protein encoded by the human gene GAS2.
Gas2 is thought to play a role in apoptosis by acting as a cell death substrate for caspases.
Gas2, a component of the microfilament system, is cleaved by a caspase (caspase-3 and caspase-7) at Asparagine 278 during apoptosis.
The cleaved form resulting from this dramatically induces the rearrangement of the Actin cytoskeleton and causes potent changes in the shape of the affected cells.
Gas2 is believed to also be involved in the membrane ruffling process.
During the G0-G1 transition phase Gas2 can be found phosphorylated on its serine residues.
Gas2 is a cytoskeleton and peripheral membrane protein that colocalizes with Actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts.
Gas2 is mainly membrane-associated but when hyperphosphorylated it will accumulate at membrane ruffles.
Gas2 is specifically expressed at growth arrest and is ubiquitously expressed with highest levels found in liver, lung and kidney.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of GAS2. Species Crossreactivity: Human, mouse, rat
