Minichromosome maintenance protein 3 (MCM3) is a protein essential for the initiation of DNA replication.
MCM3-associated protein (MCM3AP), also known as GANP or MAP80, is a 1980 amino acid protein which binds to MCM3.
MCM3AP is an acetyltransferase that acetylates MCM3, thereby playing an indirect role in DNA replication.
MCM3AP is also believed to be involved in the nuclear localization of MCM3.
Localized to the nucleus and the cytoplasm, GANP (MCM3AP) is thought to have phosphorylation-dependent DNA-primase activity that is up-regulated in germinal center regions.
MCM3AP contains a nuclear localization signal, a coiled-coil structure and four LXXLL motifs.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of GANP. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 1841-1890 of Human GANP.
