Cyclin D1 accumulates during G1 and associates with Cdk2, Cdk4 and Cdk5.
Cyclin E and Cdk2 interact during the G1 to S transition.
Cyclin G contains a typical N terminal cyclin box and a carboxy terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor).
Cyclin G expression is induced within 3 hours after growth stimulation and remains elevated with no apparent cell cycle dependency.
A serine/threonine kinase, designated GAK for cyclin G associated kinase, has been identified.
GAK has been shown to bind directly to cyclin G and to co-immunoprecipitate with Cdk5, which also associates with cyclin G.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of GAK. Species Crossreactivity: Human, mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to amino acids 101-150 of Human GAK.
