84-2337-67　［取扱停止］GADD45B (Growth arrest and DNA Damage-inducible Protein GADD45 beta, Myeloid Differentiation primary Response Protein MyD118, Negative Growth Regulatory Protein MyD118, GADD45B, MYD118) 50ul　222847

特徴

• Cellcycle progression issubject to arrest at G1 and G2 checkpointsin response to DNA damage, presumablyto allow time for DNA repair prior to entryinto S andMphase, respectively.
• The p53 tumorsuppressor isrequired for one such G1 checkpoint and functionsto upregulate expression of GADD 45 and p21.
• GADD 45 binds both Cdks and PCNA, a protein involved in DNA replication and repair.
• GADD 45 stimulates DNA excision repair in vitro and inhibits entry ofcellsinto S phase.
• Thus, it has been suggested that GADD 45 mayserve as a link between the p53-dependentcellcycle checkpoint and DNA repair.
• GADD 45-like proteins, GADD 45beta and GADD 45γ, have been shown to be induced by environmentalstresses.
• GADD 45beta and GADD 45γ are thought to induce p38/JNK activation via MEKK4 activation.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.